5ZJR
Structure of AbdB/Exd complex bound to a 'Magenta14' DNA sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.330, 49.445, 95.092 |
Unit cell angles | 90.00, 109.18, 90.00 |
Refinement procedure
Resolution | 44.906 - 3.030 |
R-factor | 0.2697 |
Rwork | 0.269 |
R-free | 0.28800 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.552 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.906 | 3.139 |
High resolution limit [Å] | 3.030 | 3.030 |
Number of reflections | 6678 | |
<I/σ(I)> | 6 | |
Completeness [%] | 98.6 | |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 90mM MgCl2, 0.1M Tris pH9.0, 22% PEG 3350 |