5ZIY

Crystal structure of Bacillus cereus FlgL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-28
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00004
Spacegroup name	C 1 2 1
Unit cell lengths	138.619, 57.986, 67.914
Unit cell angles	90.00, 114.80, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.21441
Rwork	0.212
R-free	0.24976
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.365
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.096	0.435
Number of reflections	23891	1231
<I/σ(I)>	20.2	4.4
Completeness [%]	95.4
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	15% PEG 8000, 0.1M Hepes, pH 7.4, and 0.2M zinc acetate