5ZIM

Crystal structure of bacteriorhodopsin at 1.25 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	15
Detector technology	PIXEL
Collection date	2017-10-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.8
Spacegroup name	P 63
Unit cell lengths	60.600, 60.600, 110.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.000 - 1.250
Rwork	0.125
R-free	0.14810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c3w
Data scaling software	XSCALE
Refinement software	SHELXL

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.000	47.000	1.280
High resolution limit [Å]	1.250	5.590	1.250
Rmerge	0.103	0.043	3.087
Rmeas	0.104	0.043	3.135
Number of reflections	63455	734	4679
<I/σ(I)>	21.63	82.59	1.66
Completeness [%]	100.0	99.7	100
Redundancy	33.517	35.005	33.055
CC(1/2)	1.000	0.999	0.502

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	MO, 2.0-2.5 M phosphate