5ZIL
Crystal structure of bacteriorhodopsin at 1.29 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 15 |
Detector technology | PIXEL |
Collection date | 2017-10-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.8 |
Spacegroup name | P 63 |
Unit cell lengths | 60.590, 60.590, 110.740 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.421 - 1.290 |
Rwork | 0.152 |
R-free | 0.16400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c3w |
Data scaling software | XDS |
Refinement software | SHELX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.421 | 47.421 | 1.370 |
High resolution limit [Å] | 1.290 | 3.860 | 1.290 |
Rmerge | 0.062 | 0.032 | 0.944 |
Rmeas | 0.069 | 0.035 | 1.044 |
Total number of observations | 320609 | ||
Number of reflections | 57755 | 2209 | 9249 |
<I/σ(I)> | 13.41 | 43.44 | 1.76 |
Completeness [%] | 99.9 | 99.6 | 99.7 |
Redundancy | 5.551 | 5.293 | 5.509 |
CC(1/2) | 0.999 | 0.999 | 0.481 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.6 | 293 | MO, 2.0-2.5 M PHOSPHATE |