5ZIH

Crystal structure of the red light-activated channelrhodopsin Chrimson.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	60.990, 81.440, 170.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.818 - 2.600
R-factor	0.2333
Rwork	0.228
R-free	0.27880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ug9
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmeas	0.144	0.591
Total number of observations	276798
Number of reflections	30180	4767
<I/σ(I)>	13.8	1.7
Completeness [%]	100.0	100
Redundancy	9.3	9.2
CC(1/2)	0.980	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7	298	PEG500DME, Na citrate, Na malonate, sarcosine