5ZIH
Crystal structure of the red light-activated channelrhodopsin Chrimson.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-23 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.990, 81.440, 170.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.818 - 2.600 |
| R-factor | 0.2333 |
| Rwork | 0.228 |
| R-free | 0.27880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ug9 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmeas | 0.144 | 0.591 |
| Total number of observations | 276798 | |
| Number of reflections | 30180 | 4767 |
| <I/σ(I)> | 13.8 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.3 | 9.2 |
| CC(1/2) | 0.980 | 0.595 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7 | 298 | PEG500DME, Na citrate, Na malonate, sarcosine |
