5ZIE
Crystal structure of Legionella pneumophila aminopeptidase A in complex with aspartic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-12-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.953, 102.511, 111.241 |
| Unit cell angles | 90.00, 90.52, 90.00 |
Refinement procedure
| Resolution | 33.890 - 2.000 |
| R-factor | 0.181 |
| Rwork | 0.178 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hpo |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.805 |
| Data reduction software | DENZO (2.3.1) |
| Data scaling software | SCALEPACK (2.2.0) |
| Phasing software | MOLREP (11.4.05; 02.04.2016) |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.890 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.510 |
| Number of reflections | 106641 | |
| <I/σ(I)> | 17.9 | 2.43 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1 M SPG, pH 6.0, 25 % PEG 1500 |
