5ZI9
Crystal structure of type-II LOG from Streptomyces coelicolor A3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-27 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | I 2 3 |
Unit cell lengths | 206.768, 206.768, 206.768 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 146.210 - 2.500 |
R-factor | 0.1872 |
Rwork | 0.185 |
R-free | 0.23690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wq3 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.762 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 146.210 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.108 | 0.310 |
Number of reflections | 49440 | 2396 |
<I/σ(I)> | 17.67 | 3.8 |
Completeness [%] | 98.0 | 96.8 |
Redundancy | 8.7 | |
CC(1/2) | 0.374 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Polyethylene glycol 3350, citrate |