5ZI8
Crystal structure of the PadR-family transcriptional regulator Rv3488 of Mycobacterium tuberculosis H37Rv in complex with cadmium ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-06-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.496, 49.291, 127.338 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.670 - 2.200 |
| R-factor | 0.19338 |
| Rwork | 0.191 |
| R-free | 0.23999 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zhc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.810 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.670 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.120 | 0.520 |
| Number of reflections | 9947 | |
| <I/σ(I)> | 19.17 | 2.32 |
| Completeness [%] | 95.6 | |
| Redundancy | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 296 | 0.1 M sodium acetate, 2.0 M sodium formate |
