5ZI7
Crystal structure of Legionella pneumophila aminopeptidase A in complex with glutamic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-09-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.528, 103.749, 112.691 |
| Unit cell angles | 90.00, 90.29, 90.00 |
Refinement procedure
| Resolution | 34.140 - 1.860 |
| R-factor | 0.182 |
| Rwork | 0.179 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hpo |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.864 |
| Data reduction software | DENZO (2.3.1) |
| Data scaling software | SCALEPACK (2.2.0) |
| Phasing software | MOLREP (11.4.05; 02.04.2016) |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.140 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.048 | 0.435 |
| Number of reflections | 137053 | 38086 |
| <I/σ(I)> | 17.1 | 2.7 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M SPG, pH 6.0, 25 % PEG 1500 |
