5ZI7
Crystal structure of Legionella pneumophila aminopeptidase A in complex with glutamic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-09-18 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.528, 103.749, 112.691 |
Unit cell angles | 90.00, 90.29, 90.00 |
Refinement procedure
Resolution | 34.140 - 1.860 |
R-factor | 0.182 |
Rwork | 0.179 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hpo |
RMSD bond length | 0.018 |
RMSD bond angle | 1.864 |
Data reduction software | DENZO (2.3.1) |
Data scaling software | SCALEPACK (2.2.0) |
Phasing software | MOLREP (11.4.05; 02.04.2016) |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.140 | 1.930 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.048 | 0.435 |
Number of reflections | 137053 | 38086 |
<I/σ(I)> | 17.1 | 2.7 |
Completeness [%] | 99.2 | 98.4 |
Redundancy | 3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M SPG, pH 6.0, 25 % PEG 1500 |