5ZI6
The RING domain structure of MEX-3C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-25 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.977 |
Spacegroup name | P 1 |
Unit cell lengths | 37.135, 44.567, 67.157 |
Unit cell angles | 92.43, 90.50, 91.39 |
Refinement procedure
Resolution | 36.384 - 2.200 |
R-factor | 0.2088 |
Rwork | 0.207 |
R-free | 0.24340 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.281 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXCD |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.240 |
High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
Rmerge | 0.088 | 0.052 | 0.898 |
Rmeas | 0.103 | 0.060 | 1.062 |
Rpim | 0.053 | 0.031 | 0.562 |
Number of reflections | 20938 | 1025 | 973 |
<I/σ(I)> | 4.1 | ||
Completeness [%] | 94.3 | 91.7 | 90.1 |
Redundancy | 3.6 | 3.7 | 3.1 |
CC(1/2) | 0.998 | 0.608 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2M Ammonium Acetate, 0.1M Bis-Tris pH 6.5, 25%(w/v) PEG3350 |