5ZHU
Crystal structure of the DNA-binding domain of human myelin-gene regulatory factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97778 |
| Spacegroup name | P 3 |
| Unit cell lengths | 107.414, 107.414, 48.432 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.512 - 2.202 |
| R-factor | 0.2294 |
| Rwork | 0.228 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yhu |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.562 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.512 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 31583 | |
| <I/σ(I)> | 22.4 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2M (NH4)2SO4, 0.1M HEPES pH7.0, 22.5% PEG 3350 |
