5ZHT
Crystal structure of OsD14 in complex with covalently bound KK073
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-23 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 49.349, 49.349, 192.469 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.721 - 1.532 |
R-factor | 0.1964 |
Rwork | 0.195 |
R-free | 0.22120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vxk |
RMSD bond length | 0.004 |
RMSD bond angle | 0.887 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.721 | 1.620 |
High resolution limit [Å] | 1.532 | 1.532 |
Rmerge | 0.058 | 0.396 |
Number of reflections | 78145 | |
<I/σ(I)> | 20.1 | 3.1 |
Completeness [%] | 99.5 | 97.3 |
Redundancy | 9 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG 20000, MES |