5ZHS
Crystal structure of OsD14 in complex with covalently bound KK052
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-23 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 48.970, 48.970, 192.199 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.413 - 1.490 |
R-factor | 0.1887 |
Rwork | 0.188 |
R-free | 0.21130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vxk |
RMSD bond length | 0.005 |
RMSD bond angle | 0.930 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.413 | 1.580 |
High resolution limit [Å] | 1.490 | 1.490 |
Rmerge | 0.061 | 0.390 |
Number of reflections | 84082 | |
<I/σ(I)> | 20 | 4.3 |
Completeness [%] | 99.9 | 99.2 |
Redundancy | 9.7 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 20000, HEPES |