5ZHR
Crystal structure of OsD14 in complex with covalently bound KK094
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-23 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.250, 88.640, 119.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.511 - 1.450 |
R-factor | 0.1669 |
Rwork | 0.166 |
R-free | 0.19930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vxk |
RMSD bond length | 0.009 |
RMSD bond angle | 1.215 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.511 | 1.540 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.047 | 0.274 |
Number of reflections | 173170 | |
<I/σ(I)> | 26.3 | 5.9 |
Completeness [%] | 99.0 | 94 |
Redundancy | 9.3 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG 20000, MES |