5ZHO

Human group C rotavirus VP8*s recognize type A histo-blood group antigens as ligands

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	103
Detector technology	PIXEL
Collection date	2017-11-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	37.985, 39.821, 119.814
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.789 - 1.401
R-factor	0.1428
Rwork	0.141
R-free	0.17380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zhg
RMSD bond length	0.008
RMSD bond angle	0.828
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.099	0.565
Number of reflections	36324
<I/σ(I)>	28.586
Completeness [%]	99.0
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	291	0.1 M Sodium chloride, 0.1 M BIS-TRIS pH 6.5, 1.5 M Ammonium sulfate