5ZHG

Human group C rotavirus VP8*s recognize type A histo-blood group antigens as ligands

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	Xenocs GeniX 3D Cu HF
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2017-08-31
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	38.065, 40.070, 124.014
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.129 - 1.799
R-factor	0.181
Rwork	0.180
R-free	0.20400
RMSD bond length	0.004
RMSD bond angle	0.697
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.799	1.800
Rmerge	0.099	0.565
Number of reflections	18290
<I/σ(I)>	21.019
Completeness [%]	99.4
Redundancy	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	291	0.1 M Sodium chloride, 0.1 M BIS-TRIS pH 6.5, 1.5 M Ammonium sulfate