5ZH6
Crystal structure of Parvalbumin SPV-II of Mustelus griseus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 190 |
| Detector technology | PIXEL |
| Collection date | 2016-04-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.870, 41.800, 60.340 |
| Unit cell angles | 90.00, 110.41, 90.00 |
Refinement procedure
| Resolution | 33.615 - 1.540 |
| R-factor | 0.1953 |
| Rwork | 0.194 |
| R-free | 0.21250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zgm |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.228 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmeas | 0.086 | 0.201 |
| Number of reflections | 30489 | 1776 |
| <I/σ(I)> | 16.22 | 6.33 |
| Completeness [%] | 97.5 | 77.4 |
| Redundancy | 5.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 2.3 M Ammonium Sulfate, 5% Ethylene Glycol, 0.1 mM HEPES pH 7.0 |
