5ZGM
Crystal Structure of Parvalbumin SPVI, the Major Allergens in Mustelus griseus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 190 |
Detector technology | PIXEL |
Collection date | 2016-04-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 1 |
Unit cell lengths | 26.207, 43.289, 52.570 |
Unit cell angles | 65.97, 88.93, 88.61 |
Refinement procedure
Resolution | 39.530 - 1.399 |
R-factor | 0.1631 |
Rwork | 0.162 |
R-free | 0.18560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5pal |
RMSD bond length | 0.005 |
RMSD bond angle | 0.713 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.420 |
High resolution limit [Å] | 1.399 | 1.400 |
Rmeas | 0.117 | 0.848 |
Rpim | 0.060 | 0.447 |
Number of reflections | 37631 | 1884 |
<I/σ(I)> | 25.15 | 3.34 |
Completeness [%] | 90.9 | 90.9 |
Redundancy | 3.6 | 3.2 |
CC(1/2) | 0.712 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 2.5 M Ammonium Citrate, 0.1 M HEPES pH 7.0 |