5ZG8
Crystal Structure of TtNRS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.976 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 67.294, 67.294, 189.117 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 2.440 - 2.400 |
R-factor | 0.1579 |
Rwork | 0.155 |
R-free | 0.20860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1x54 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.761 | 2.440 |
High resolution limit [Å] | 2.399 | 2.399 |
Rmerge | 0.105 | 0.571 |
Number of reflections | 19150 | 993 |
<I/σ(I)> | 7.48 | |
Completeness [%] | 94.7 | |
Redundancy | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M Ammonium sulfate, 0.1M Sodium cacodylate trihydrate pH 6.5, 30%(w/v) Polyethylene glycol 8,000 |