5ZFQ

Crystal structure of PilT-4, a retraction ATPase motor of Type IV pilus , from Geobacter sulfurreducens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-02-26
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	H 3 2
Unit cell lengths	181.760, 181.760, 123.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.720 - 2.600
R-factor	0.1934
Rwork	0.191
R-free	0.24360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jvu
RMSD bond length	0.010
RMSD bond angle	1.234
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	97.198	22.720	2.740
High resolution limit [Å]	2.600	8.220	2.600
Rmerge		0.027	0.623
Rmeas	0.110	0.029	0.680
Rpim	0.042	0.011	0.267
Number of reflections	23914	755	3439
<I/σ(I)>	12.6	20.7	1.2
Completeness [%]	99.2	94	98.6
Redundancy	6.4	6.4	6.1
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.0M succinic acid pH7.0