5ZFQ
Crystal structure of PilT-4, a retraction ATPase motor of Type IV pilus , from Geobacter sulfurreducens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ DW |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-02-26 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 2 |
Unit cell lengths | 181.760, 181.760, 123.570 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.720 - 2.600 |
R-factor | 0.1934 |
Rwork | 0.191 |
R-free | 0.24360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jvu |
RMSD bond length | 0.010 |
RMSD bond angle | 1.234 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER (2.5.7) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 97.198 | 22.720 | 2.740 |
High resolution limit [Å] | 2.600 | 8.220 | 2.600 |
Rmerge | 0.027 | 0.623 | |
Rmeas | 0.110 | 0.029 | 0.680 |
Rpim | 0.042 | 0.011 | 0.267 |
Number of reflections | 23914 | 755 | 3439 |
<I/σ(I)> | 12.6 | 20.7 | 1.2 |
Completeness [%] | 99.2 | 94 | 98.6 |
Redundancy | 6.4 | 6.4 | 6.1 |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.0M succinic acid pH7.0 |