5ZFK

UDP Glucose alpha tetrahydrobiopterin glycosyltransferase from Synechococcus species PCC 7942 - UDP-BH2 complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979,0.979,0.987
Spacegroup name	C 1 2 1
Unit cell lengths	168.370, 79.984, 53.827
Unit cell angles	90.00, 90.96, 90.00

Refinement procedure

Resolution	50.000 - 1.750
R-factor	0.16072
Rwork	0.159
R-free	0.19987
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ze7
RMSD bond length	0.019
RMSD bond angle	2.039
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.750	1.750
Number of reflections	71852
<I/σ(I)>	14.5
Completeness [%]	99.2
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Bis-tris, PEG3350