5ZF2
Crystal structure of Trxlp from Edwardsiella tarda EIB202
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97852 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 34.273, 34.273, 264.702 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.059 - 1.980 |
R-factor | 0.181 |
Rwork | 0.177 |
R-free | 0.21540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yzu |
RMSD bond length | 0.009 |
RMSD bond angle | 0.923 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.000 |
High resolution limit [Å] | 1.970 | 5.350 | 1.970 |
Rmerge | 0.076 | 0.048 | 0.200 |
Rmeas | 0.078 | 0.049 | 0.203 |
Rpim | 0.014 | 0.010 | 0.037 |
Number of reflections | 7481 | 499 | 351 |
<I/σ(I)> | 8.1 | ||
Completeness [%] | 99.9 | 99.8 | 99.7 |
Redundancy | 30.4 | 25.3 | 29.3 |
CC(1/2) | 0.999 | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 30% PEG 8000, 100mM sodium acetate, pH6.5, 200mM lithium sulfate |