5ZF0
X-ray Structure of the Electron Transfer Complex between Ferredoxin and Photosystem I
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-21 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 214.101, 239.668, 265.605 |
| Unit cell angles | 90.00, 101.08, 90.00 |
Refinement procedure
| Resolution | 158.040 - 4.200 |
| R-factor | 0.35423 |
| Rwork | 0.353 |
| R-free | 0.37705 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jb0 |
| Data reduction software | iMOSFLM (1.0.7) |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER (2.5.5) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 158.040 | 4.430 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rmerge | 0.159 | 0.868 |
| Number of reflections | 191480 | 212827 |
| <I/σ(I)> | 8.7 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 277 | 41% PEG200, 100 mM acetate buffer, 100 mM NaCl |
