5ZER
UDP Glucose alpha tetrahydrobiopterin glycosyltransferase from Synechococcus species PCC 7942 - BH2 complex form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-28 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 167.130, 80.000, 53.380 |
Unit cell angles | 90.00, 91.27, 90.00 |
Refinement procedure
Resolution | 45.710 - 2.390 |
R-factor | 0.21881 |
Rwork | 0.215 |
R-free | 0.28580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ze7 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.551 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.630 |
High resolution limit [Å] | 2.390 | 2.590 |
Number of reflections | 26482 | |
<I/σ(I)> | 8.4 | |
Completeness [%] | 98.4 | |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | Bis-tris, PEG3350, Galactose |