5ZE4
The structure of holo- structure of DHAD complex with [2Fe-2S] cluster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-17 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.97774 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 135.467, 135.467, 65.978 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.110 |
| R-factor | 0.17479 |
| Rwork | 0.173 |
| R-free | 0.21519 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j83 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.191 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.189 | 1.240 |
| Number of reflections | 36139 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 25.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 289 | 0.1 M sodium acetate pH 5.0, 1.5 M ammonium sulfate |
