5ZDS
Crystal structure of the second PDZ domain of Frmpd2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97858 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 48.176, 56.842, 32.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.2017 |
Rwork | 0.198 |
R-free | 0.23470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vj6 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.831 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.640 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmeas | 0.065 | 0.268 |
Number of reflections | 12699 | 1248 |
<I/σ(I)> | 22.52 | 7.7 |
Completeness [%] | 99.7 | 100 |
Redundancy | 6.3 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG3350 20%, sodium thiocyanate 0.2M |