5ZDP
Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with ferulenol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 259.501, 63.109, 138.273 |
| Unit cell angles | 90.00, 122.21, 90.00 |
Refinement procedure
| Resolution | 39.320 - 2.710 |
| R-factor | 0.195 |
| Rwork | 0.192 |
| R-free | 0.24852 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vv9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.404 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.076 | 0.644 |
| Number of reflections | 52186 | 2576 |
| <I/σ(I)> | 7.8 | 1.7 |
| Completeness [%] | 98.8 | 98.5 |
| Redundancy | 2.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 30% PEG400, 0.1M IMIDAZOLE, 0.5M POTASSIUM FORMATE |
