5ZDN
The complex structure of FomD with CDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-21 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 56.165, 77.452, 96.566 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.280 - 2.020 |
R-factor | 0.19559 |
Rwork | 0.193 |
R-free | 0.24750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3exm |
RMSD bond length | 0.019 |
RMSD bond angle | 2.044 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.020 | 2.020 |
Rmerge | 0.068 | 0.525 |
Number of reflections | 14166 | 1015 |
<I/σ(I)> | 16.4 | 2.1 |
Completeness [%] | 99.8 | 97.4 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 278 | Tris-HCl, magnesium chloride, PEG 8000, EtOH, CDP |