5ZDB
Crystal structure of poly(ADP-ribose) glycohydrolase (PARG) from Deinococcus radiodurans in complex with ADP-ribose (P21)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.290, 97.427, 59.369 |
| Unit cell angles | 90.00, 93.65, 90.00 |
Refinement procedure
| Resolution | 25.959 - 1.972 |
| R-factor | 0.1577 |
| Rwork | 0.155 |
| R-free | 0.19670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zda |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.922 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.959 | 2.040 |
| High resolution limit [Å] | 1.972 | 1.970 |
| Rmerge | 0.086 | 0.502 |
| Number of reflections | 33944 | |
| <I/σ(I)> | 14.353 | 2.019 |
| Completeness [%] | 98.2 | 91.1 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 283 | 0.1 M Sodium citrate tribasic dihydrate pH 6.0, 10% 2-Propanol, 10% Polyethylene glycol 4000 |
