5ZCT
The crystal structure of the poly-alpha-L-glutamate peptides synthetase RimK at 2.05 angstrom resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-29 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 212.618, 121.628, 119.741 |
| Unit cell angles | 90.00, 118.74, 90.00 |
Refinement procedure
| Resolution | 48.213 - 2.050 |
| R-factor | 0.1983 |
| Rwork | 0.196 |
| R-free | 0.23980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iwx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.067 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 4.420 | 2.050 |
| Rmerge | 0.073 | 0.046 | 0.550 |
| Rmeas | 0.080 | 0.050 | 0.629 |
| Rpim | 0.033 | 0.020 | 0.299 |
| Total number of observations | 875207 | ||
| Number of reflections | 164792 | 16929 | 16418 |
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 98.7 | 99.9 | 98.8 |
| Redundancy | 5.3 | 6.4 | 4.1 |
| CC(1/2) | 0.998 | 0.557 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM Bis-Tris propane (pH 7.0), 210 mM Na2SO4, and 16% PEG3350 |
