5ZCG
Crystal structure of OsPP2C50 S265L/I267V:OsPYL/RCAR3 with (+)-ABA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 |
Unit cell lengths | 70.738, 76.351, 77.942 |
Unit cell angles | 62.96, 72.04, 66.93 |
Refinement procedure
Resolution | 32.946 - 2.100 |
R-factor | 0.2028 |
Rwork | 0.202 |
R-free | 0.23730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gwp |
RMSD bond length | 0.004 |
RMSD bond angle | 0.931 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.950 | 2.041 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.068 | 0.332 |
Rpim | 0.046 | 0.221 |
Number of reflections | 89408 | 7413 |
<I/σ(I)> | 16.61 | |
Completeness [%] | 95.8 | |
Redundancy | 3.2 | |
CC(1/2) | 0.996 | 0.912 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 7.5% PEG 3350, 80mM ammonium thiocyanate |