5ZCD
Crystal structure of Alpha-glucosidase in complex with maltotriose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-19 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.628, 84.745, 128.514 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.504 - 1.707 |
R-factor | 0.1792 |
Rwork | 0.178 |
R-free | 0.20550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ze0 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.041 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.107 | 0.675 |
Rmeas | 0.117 | 0.743 |
Number of reflections | 66627 | 10452 |
<I/σ(I)> | 11.1 | 2 |
Completeness [%] | 99.6 | 97.8 |
Redundancy | 6.6 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | PEG 3350, Calcium Chloride |