5ZC4
Crystal Structure of RNF13 RING domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-18 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97947 |
Spacegroup name | P 62 |
Unit cell lengths | 90.338, 90.338, 45.445 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.619 - 1.906 |
R-factor | 0.1671 |
Rwork | 0.166 |
R-free | 0.18580 |
Structure solution method | SIRAS |
RMSD bond length | 0.015 |
RMSD bond angle | 1.431 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXD |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.170 | 1.950 |
High resolution limit [Å] | 1.906 | 1.906 |
Rmerge | 0.051 | 0.813 |
Rpim | 0.018 | 0.298 |
Number of reflections | 16628 | 1071 |
<I/σ(I)> | 24.6 | 2.3 |
Completeness [%] | 99.4 | 100 |
Redundancy | 8.8 | 7.9 |
CC(1/2) | 0.999 | 0.793 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100% Tacsimate |