5ZC3
The Crystal Structure of PcRxLR12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.532, 78.308, 179.057 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.000 - 3.005 |
R-factor | 0.2121 |
Rwork | 0.209 |
R-free | 0.26030 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.066 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 19409 | 1622 |
<I/σ(I)> | 32.3 | |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 7.1 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 100mM HEPES pH 7.5, 6%(w/v) PEG 6000 and 5%(v/v) -2-methyl-2,4-pentanediol |