5ZBN
Structure of glycolate oxidase without FMN from Nicotiana benthamiana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-15 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.1 |
| Spacegroup name | I 4 |
| Unit cell lengths | 145.351, 145.351, 156.904 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.320 |
| R-factor | 0.19654 |
| Rwork | 0.196 |
| R-free | 0.21466 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zbm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.311 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.094 | 0.570 |
| Number of reflections | 70355 | 3525 |
| <I/σ(I)> | 27.2 | |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 13.1 | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 10%(w/v) polyethylene glycol 3000, 0.1M Na/K phosphate |
