5ZBL

Crystal structure of type-I LOG from Corynebacterium glutamicum in complex with AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-21
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	70.153, 70.714, 190.802
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	95.400 - 2.300
R-factor	0.1994
Rwork	0.196
R-free	0.25920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5its
RMSD bond length	0.013
RMSD bond angle	1.627
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.400	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.102	0.394
Rpim	0.033	0.157
Number of reflections	41734	2002
<I/σ(I)>	37.3
Completeness [%]	96.8
Redundancy	6.5
CC(1/2)		0.671

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	ethylene glycol, sodium phosphate dibasic titrated with citric acid to pH 4.2, ammonium sulfate