5ZB1
Monomeric crystal structure of orf57 from KSHV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.2724 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 175.782, 175.782, 175.782 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.980 - 3.061 |
| R-factor | 0.2251 |
| Rwork | 0.223 |
| R-free | 0.26930 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.131 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.060 | 3.060 |
| Rmerge | 0.109 | 0.525 |
| Rmeas | 0.109 | 0.528 |
| Number of reflections | 9118 | 440 |
| <I/σ(I)> | 112.3 | 21.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 82.1 | 85.8 |
| CC(1/2) | 0.995 | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1M Bis-Tris propane pH 7.0, 0.8M Lithium sulfate |
