5ZAT
Crystal structure of 5-carboxylcytosine containing decamer dsDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 180 |
Detector technology | PIXEL |
Collection date | 2016-06-13 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.9676 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 22.465, 36.333, 30.215 |
Unit cell angles | 90.00, 100.17, 90.00 |
Refinement procedure
Resolution | 29.740 - 1.060 |
R-factor | 0.1573 |
Rwork | 0.156 |
R-free | 0.18140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.802 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.100 |
High resolution limit [Å] | 1.060 | 1.060 |
Number of reflections | 21838 | |
<I/σ(I)> | 26.12 | |
Completeness [%] | 98.0 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 50mM sodium succinat pH 5.5, 20mM MgCl2, 0.5mM spermine, 3.0 ammonium sulfate |