5ZAS
Crystal structure of 5-formylcytosine containing decamer dsDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 180 |
Detector technology | PIXEL |
Collection date | 2016-06-13 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.9676 |
Spacegroup name | P 61 |
Unit cell lengths | 54.548, 54.548, 45.819 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.240 - 1.560 |
R-factor | 0.14736 |
Rwork | 0.147 |
R-free | 0.16210 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.338 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.620 |
High resolution limit [Å] | 1.560 | 1.560 |
Number of reflections | 11176 | |
<I/σ(I)> | 37.6 | |
Completeness [%] | 99.5 | |
Redundancy | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 50mM cacodylate pH 6.0, 20mM magnesium acetate, 0.5mM spermine, 100mM NaCl, 25% MPD |