5ZAC

Crystal structure of ConA-R2M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9716
Spacegroup name	P 21 21 21
Unit cell lengths	64.227, 119.893, 125.211
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.590
R-factor	0.28303
Rwork	0.278
R-free	0.37192
RMSD bond length	0.013
RMSD bond angle	1.686
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.590
<I/σ(I)>	18
Completeness [%]	94.4
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	300	each 0.2 mM ConA in 20 mM HEPES, 100 mM NaCl, 5 mM CaCl2, 5 mM MnCl2 pH 7.2