5ZA3
Structure of a C-terminal S. mutans response regulator VicR domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 277.15 |
| Detector technology | CCD |
| Collection date | 2013-04-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.647, 83.984, 39.978 |
| Unit cell angles | 90.00, 108.00, 90.00 |
Refinement procedure
| Resolution | 20.442 - 1.500 |
| R-factor | 0.1987 |
| Rwork | 0.198 |
| R-free | 0.22160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zxj |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.019 |
| Data reduction software | HKL-2000 (v706) |
| Data scaling software | HKL-2000 (v706) |
| Phasing software | PHENIX (1.8.4) |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.065 | |
| Number of reflections | 35819 | |
| <I/σ(I)> | 35.388 | |
| Completeness [%] | 90.0 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 9 | 298 | 100 mM BICINE, pH 9.0, 20% polyethylene glycerol 6000 |
