5ZA0
A cryo-protectant induces the conformational change of glyceraldehyde-3-phosphate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 121.295, 121.295, 156.056 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.880 - 2.000 |
| R-factor | 0.18485 |
| Rwork | 0.184 |
| R-free | 0.20474 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s7c |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.284 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.880 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 39522 | |
| <I/σ(I)> | 21.8 | |
| Completeness [%] | 99.6 | |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277.15 | Ammonium sulfate, MES |
