5Z9Z

The C-terminal RRM domain of Arabidopsis SMALL RNA DEGRADING NUCLEASE 1 (E329A/E330A/E332A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2013-04-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
Unit cell lengths	41.132, 65.620, 74.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.851 - 2.049
R-factor	0.1807
Rwork	0.178
R-free	0.22700
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.579
Data reduction software	HKL-2000 (0.98.699a)
Data scaling software	HKL-2000 (0.98.699a)
Phasing software	PHENIX (1.8.2_1309)
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.960	2.120
High resolution limit [Å]	2.049	2.050
Number of reflections	6556
<I/σ(I)>	29.4
Completeness [%]	99.6
Redundancy	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	PEG 3350, ammonium citrate