5Z9O
The crystal structure of Cyclopropane-fatty-acyl-phospholipid synthase from Lactobacillus acidophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-12-11 |
Detector | Nonius Kappa CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 120.982, 120.982, 163.487 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.106 - 2.700 |
R-factor | 0.17696 |
Rwork | 0.175 |
R-free | 0.21258 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l1e |
RMSD bond length | 0.013 |
RMSD bond angle | 1.814 |
Data reduction software | PDBSET |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.210 | 2.773 |
High resolution limit [Å] | 2.700 | 2.703 |
Number of reflections | 37057 | |
<I/σ(I)> | 5.2 | |
Completeness [%] | 99.9 | |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 1.5 M ammonium sulfate, 0.1 M potassium sodium tartrate tetrahydrate, 0.1 M sodium citrate tribasic dehydrate |