5Z9H

Crystal structure of KAI2_ply2(A219V)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93.1
Detector technology	IMAGE PLATE
Collection date	2012-03-14
Detector	RIGAKU RAXIS IV
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	50.842, 55.494, 53.019
Unit cell angles	90.00, 116.42, 90.00

Refinement procedure

Resolution	27.747 - 1.490
R-factor	0.1358
Rwork	0.134
R-free	0.16800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wom
RMSD bond length	0.006
RMSD bond angle	1.028
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.520
High resolution limit [Å]	1.490	1.490
Number of reflections	43147
<I/σ(I)>	48.75
Completeness [%]	99.6
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	1M Na-citrate, 100 mM HEPES (pH 7.5), 5% glycerol