5Z9G
Crystal structure of KAI2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 93.1 |
Detector technology | IMAGE PLATE |
Collection date | 2012-03-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.599, 55.785, 53.034 |
Unit cell angles | 90.00, 116.07, 90.00 |
Refinement procedure
Resolution | 47.640 - 1.490 |
R-factor | 0.13854 |
Rwork | 0.136 |
R-free | 0.17662 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wom |
RMSD bond length | 0.023 |
RMSD bond angle | 1.929 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.520 |
High resolution limit [Å] | 1.490 | 1.490 |
Number of reflections | 43387 | |
<I/σ(I)> | 54.1 | |
Completeness [%] | 99.7 | |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 290 | 1M Na-citrate, 100 mM HEPES (pH 7.5), 5% glycerol |