5Z8S

Human Mitochondrial ferritin mutant - C102A/C130A/E27C/E61C/E62C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9789
Spacegroup name	F 4 3 2
Unit cell lengths	182.736, 182.736, 182.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.923 - 1.973
R-factor	0.1676
Rwork	0.166
R-free	0.19950
RMSD bond length	0.006
RMSD bond angle	0.799
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.923	2.044
High resolution limit [Å]	1.973	1.973
Number of reflections	18997
<I/σ(I)>	5.4
Completeness [%]	100.0
Redundancy	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	0.1M Tris-HCl, 3.4M 1,6-Hexanediol