5Z8B
Truncated N-acetylglucosaminyl transferase KfiA from E. coli K5 strain apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-19 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.409, 70.862, 147.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.710 - 2.910 |
R-factor | 0.18227 |
Rwork | 0.178 |
R-free | 0.26517 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.588 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.710 | 3.080 |
High resolution limit [Å] | 2.910 | 2.910 |
Number of reflections | 16958 | |
<I/σ(I)> | 8.9 | |
Completeness [%] | 99.5 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | PEG 3350 |