5Z88

Crystal structure of H5 hemagglutinin G228S mutant with 3SLN from A/chicken/Taiwan/0502/2012

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	110.227, 110.227, 314.193
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.121 - 3.000
R-factor	0.2174
Rwork	0.214
R-free	0.29270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yt9
RMSD bond length	0.010
RMSD bond angle	1.194
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.080	0.506
Rmeas	0.094	0.605
Rpim	0.049	0.324
Number of reflections	13555
<I/σ(I)>	13.3
Completeness [%]	98.6
Redundancy	3.5	3.3
CC(1/2)	0.966	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M Tris, 0.2M MgCl, 16%PEG8000