5Z87
Structural of a novel b-glucosidase EmGH1 at 2.3 angstrom from Erythrobacter marinus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-15 |
Detector | RAYONIX MX325HE |
Wavelength(s) | 0.97930 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.640, 132.150, 194.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.320 - 2.300 |
R-factor | 0.1642 |
Rwork | 0.162 |
R-free | 0.21160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u48 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.857 |
Data reduction software | CrystalClear |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmeas | 0.138 | 0.753 |
Rpim | 0.037 | 0.200 |
Number of reflections | 99834 | 4956 |
<I/σ(I)> | 22.7 | 3.87 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14 | 14.1 |
CC(1/2) | 0.938 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% PEG 3350, 0.05M magnesium acetate, 2% benzamidine hydrochloride |